Material resources and infrastructure

We are equipped with the last tecnology:

  • Agilent Technologies – Agilent 8453 UV-Visible Spectrophotometer
  • Agilent Technologies – 1260 Infinity preparative HPLC system with dual pump, temperature-controlled dual loop autosampler, Diode Array Detector and temperature-controlled fraction collector.
  • Aligent Technologies – 4 Hewlett-Packard HPLC machines, equipped with DAD and RID detectors.
  • Applied Biosystems – API 3200 Qtrap mass spectrometer with electrospray ionisation (ESI) source and a triple quadrupole mass spectrometer / linear ion trap.
  • ThermoFisher Scientific – LTQ-Orbitrap Velos mass spectrometer
  • – LS 45 Fluorescence Spectrometer
  • Malvern Instruments – Particle and Potential Z Zetasizer Nano S analyzer
  • – Mini-PROTEAN Tetra Cell Electrophoresis system (BioRad)
  • Omega BMG-Labtech – Microplate reader equipped with UV-Visible Spectrophotometer
  • – Incubator cells, Memmert CO2 incubator INCO 108
  • – Other equipment (Freeze dryer, Centrifuge, Evaporator, …)
  • Agilent Technologies – Agilent 8453 UV-Visible Spectrophotometer (Agilent Technologies).
  • Agilent Technologies – 1260 Infinity preparative HPLC system (Agilent Technologies) with dual pump, temperature-controlled dual loop autosampler, Diode Array Detector and temperature-controlled fraction collector.
  • Aligent Technologies – 4 Hewlett-Packard HPLC machines (Agilent Technologies), equipped with DAD and RID detectors.

Material resources and infrastructure

University of Salamanca

  • Agilent 8453 UV-Visible Spectrophotometer (Agilent Technologies).
  • 1260 Infinity preparative HPLC system (Agilent Technologies) with dual pump, temperature-controlled dual loop autosampler, Diode Array Detector and temperature-controlled fraction collector.
  • 4 Hewlett-Packard HPLC machines (Agilent Technologies), equipped with DAD and RID detectors.
  • API 3200 Qtrap mass spectrometer (Applied Biosystems) with electrospray ionisation (ESI) source and a triple quadrupole mass spectrometer / linear ion trap.
  • LTQ-Orbitrap Velos mass spectrometer (ThermoFisher Scientific)
  • LS 45 Fluorescence Spectrometer (PerkinElmer)
  • Mini-PROTEAN Tetra Cell Electrophoresis system (BioRad)
  • Stereoscopic fluorescence microscope: M205 FA (Leica)
  • Refrigerated Incubators: FTC90i (VELP scientifica) and IPP SS (Memmert)
  • Laminar flow cabinet: AV-30/70 (Telstar)
  • Autoclave: Presoclave-II (Selecta)
  • Centrifugal evaporator: micVac (GeneVac)
  • Microplate reader: Tecan
  • Other equipment (Freeze dryer, Centrifuge, Evaporator, …)
  • Other equipment available: Access to the services offered by the NUCLEUS platform of the University of Salamanca (https://nucleus.usal.es/)

University of Porto, Faculty of Science

  • FlexStation 3 Multi-Mode Microplate Reader
  • Horiba Fluoromax-4 fluorimeter
  • HPLC-DAD
  • HPLC-MS
  • UHPLC-DAD/FLD
  • HPLC-RI
  • Electric cell-substrate impedance sensing (ECIS)
  • Cell culture laboratory
  • Platforms belonging to the General Services of the institution:: MALDI-TOF, LTQ-Orbitrap XL mass spectrometer, NMR

University of Porto, Faculty of Pharmacy

  • 2 Laminar flow chambers
  • 2 Incubators with temperature and CO2 control
  • 3 Water baths4 Centrifuges2 Microscopes
  • 1 Suction system
  • 4 Refrigerators
  • 2 freezers (-80 ◦C)
  • 1 freezer (-20 ◦C)
  • 2 UV / VIS microplate spectrophotometers1 microplate fluorimeter
  • 4 HPLC (HPLC-DAD/HPLC-UV)

Polytechnic institute of Bragança

  • Pressure reactorMicrowave reactorSynthesis reactorAtomization and microencapsulation system
  • Ultrasonic probeSimulated moving bed chromatography equipment
  • Analytical HPLC equipment with UV detector and polarimeter
  • HPLC-preparative equipment with UV, DSC, TG detectors GC-MS with automatic thermal desorption
  • HPLC-DAD with autosampler and fraction collector
  • HPLC with UV-vis detector, RI and fluorescence detector
  • UPLC-DAD-ESI/MSn coupled to FL, GC-FID
  • UV-vis Spectrophotometer
  • Flow cytometer with Samsung monitor 24″
  • CO2 Incubator
  • Refrigerated centrifugesMicroplate reader (fluorescence)
  • Ultra-Low Temperature Freezer Upright 400 (-86ºC)
  • Freezer (-80ºC)
  • Freeze dried
  • Microcentrifuge
  • SSpray dryer
  • Particle size analyzerUltrasonic bathUV-vis spectrophotometerRotary evaporator with G3 condenserHybrid water production system HPLC/UV
  • Reactor for hydrothermal treatments
  • Extraction / reaction system
  • Soxhlet extractor, laboratory scale and pilot
  • Protein determination system (Kjeldahl)
  • Laboratory mill

University of Vigo

  • Equipment for the study of watersheds (level or flow sensors, electrical conductivity, temperature, automatic sampling, pluviometers…)
  • Equipment for the study of soils (spectrophotometer of absorption / atomic emission with hydride generator, mercury analyser, segmented flow analyzer, titrometer…).
  • GC-MS equipment (single quadrupole, triple quadruple and ITD)
  • HPLC equipment with photodiode detection (HPLC-DAD) or fluorescence
  • Triple quadrupole mass spectrometry equipment (HPLC-QqQ)
  • Flash chromatography
  • HPLC-semiprep.
  • PCR equipment
  • Gel electrophoresis FPLC
  • Capillary electrophoresis (CE)
  • Spectrofluorimeter FastPrep homogenizer
  • FTIR / UV-Vis / Fluorescence high performance spectrophotometers
  • Zetasizer Z-Particle and Potential Analyzer
  • BET, and preparation of nanoparticles with nano spray drier and electrospinning

University of Valladolid

Center

  • Experimental winery with a capacity for 30,000 kg of grapes
  • Barrel room
  • Bottle room
  • Tasting room with 45 seats

Own of the group

  • Micro-oxygenation room with tanks for micro-oxygenation and active and passive micro-oxygenation systems
  • Barrel room provided with barrels of different oaks, volumes, coopers
  • Laboratory of chemical analysis of musts and wines equipped with HPLC-DAD, GC-FID, GC-ECD, UV-ViS spectrophotometers, equipment for the preparation of samples
  • Oxygen measurement laboratory (nanotraces of dissolved oxygen)
  • Equipments for measuring the oxygen permeability of materials
  • Equipment for measuring and analyzing images

Central services

  • Chromatography unit
  • Mass spectrometry unit
  • RMN
  • RX
  • Inorganic elemental analysis
  • Magnetic resonance imaging unit  (RMI)

University of Coimbra

Full availability:

  • High performance gas chromatography system coupled to mass detector (quadrupole) – CG / MS Agilent 6890/5973 High performance gas chromatography system coupled to FID detector – CG/FID Agilent 6890.
  • High performance liquid chromatography system coupled to PDA detector – HPLC-PDA GILSON
  • liquid chromatography system, Prep coupled to UV-V detector and fraction collector –Flash Chromatography BUCHI
  • Spectrophotometer – NanoDrop ND-1000
  • Image treatment system for gel and blot – GE Healthcare Typhoon FLA 9000 CO2 incubator for cell culture – Thermo Scientific Series II Model 3111
  • • Laminar flow cabinet for cell culture – Nuaire Class II type B2
  • Real-time PCR equipment – Bio-Rad IQTM5 Thermocycler – Bio-Rad C1000 TouchTM SLT – ELISA
  • High performance liquid chromatography system with UV-Visible detector – HPLC-UV / VIS GILSONHigh performance gas chromatography system coupled to mass spectrometry detector (cuadrupole) – CG/MS Agilent 6890/5973
  • Liquid chromatography system with mass detector – Agilent 1100 LC-MS / MS / Applied Biosystem API 3000 Spectrophotometer – Thermo Electron Corporation Evolution Optics 600

Other equipment available upon payment:

  • Liquid chromatography system coupled to photodiode detector and mass spectrometer HPLC-PDA-ESI / MSn Thermo Finnigan – QITMS LCQ Advantage Ion Max
  • Flow cytometer – Becton Dickinson – 4-Color FACSCalibur
  • Confocal Microscope– Zeiss LSM 510 Meta

University of Tras-os-Montes and Alto Douro

  • HPLC-DAD-FLD equipment
  • HPLC-DAD (Thermo)
  • Refrigerated centrifuge equipped with rotors for microtubes and for 50 ml tubes (Sigma)HPLC
  • GC-MC
  • GC-FID
  • Ion exchange chromatographFTIR spectrometer with ATR and DRIFT (Thermo)Citometer
  • Hyperspectral cameras for the analysis of food compositionFreeze-dryer
  • Rheometer
  • Alveograph
  • 3D volume analyzerTexture analyzer – Rapid Visco AnalyzeTexturometer
  • Laboratory of sensory analysis
  • Climatic chambers
  • PCR
  • Installations enabled to carry out animal testing

Matarromera Winery

Different production centers:

  • Bodega Matarromera (winery) since 1.988, in Valbuena de Duero, D.O. Ribera de Duero wines.
  • Bodega Emina Ribera (winery) since 2.005 in Valbuena de Duero,  D.O. Ribera de Duero wines.
  • Bodega Valdelosfrailes (winery) since 1.998 in Cubillas de Santa Marta, D.O. Cigales wines.
  • Destilerías del Duero (distillery), since 2.003 in Valbuena de Duero liquors and spirits.
  • Bodega Emina Rueda (winery) since 2.007 in Medina del Campo, D.O. Rueda.
  • Molecular Deconstruction Center since 2.011, production of wines with low alcohol content in Valbuena de Duero.

Resources available to the IBERPHENOL consortium:

  • Tanks of 25.000, 50.000, 10.000L, Isothermal tanks, experimental tanks 100L, etc.
  • French and American oak barrels, Flexible impeller pumps, piston transfer pump, peristaltic pump for filling barrels
  • Cooling equipment and cold-water tank Hot water tank
  • Water pump
  • Overpressure valves
  • Cold-heat automation Earth Filters
  • Tangential filter and amicrobic filtration bench (microfiltration)
  • Weighing scales, vintage reception hopper, destemmer-crusher, vintage peristaltic pumps, SO2 dispensers, pneumatic press (N2) Reverse osmosis facilities Micro-oxygenation equipment Hot water tanks Hydraulic forklift equipment currently available in the central laboratory of Matarromera Winery:
  • HPLC with DAD and fluorescence detectors.
  • ORAC Spectrofluorimeter (measure of antioxidant capacity)
  • Spectrophotometer Milliflex PLUS equipment (microbiology)
  • Water purification equipment
  • OenoFOSS. Infrared spectroscopy multi-analyzer
  • Rotavapor
  • Ultrasonic equipment
  • Cromoenos
  • Centrifuges (eppendorf and falcon)
  • pH meter
  • Vacuum pump
  • Another series of basic equipment (precision weighing scales, thermal bath, heating mantle, ebullometer, distillation systems …)
  • Column with rotating cones for dealcoholization, etc.
  • In addition, in the support laboratories, there are other equipment such as the gas chromatograph with FID detector or the Dyostem.

Matarromera, S.L currently has 2 plants for the extraction of antioxidant compounds and a desalcoholizer for tests of partial and total reduction of the alcohol content in wines, reusing the residual ethanol in the polyphenol extraction plant.